CREB-binding protein (CBP) gene family regulates planarian survival and stem cell differentiation

In developmental biology, the regulation of stem cell plasticity and differentiation remains an open question. CBP(CREB-binding protein)/p300 is a conserved gene family that functions as transcriptional co-activator plays important roles in wide range cellular processes, including death, DNA damage response, tumorigenesis. The acetyl transferase activity CBPs particularly important, histone non-histone acetylation results changes chromatin architecture protein affect expression. Many studies have described CBP/p300 proliferation differentiation. planarian Schmidtea mediterranea excellent model for vivo study molecular mechanisms underlying during regeneration. However, how this process regulated genetically epigenetically not well-understood yet. We identified 5 distinct Smed-cbp genes S. show different expression patterns. Functional analyses revealed Smed-cbp-2 appears to be essential maintenance. On other hand, silencing Smed-cbp-3 resulted growth blastemas were apparently normal, but remained largely unpigmented undifferentiated. also affected several lineages neural, epidermal, digestive, excretory types. Finally, we analysed predicted interactomes CBP-2 CBP-3 initial step better understand their biology. Our indicate cbp play key maintenance